This is an update on Orion, the
Kemp's ridley sea turtle with pneumonia. I last
reported about Orion in January. I am happy to say that Orion began to feel better after starting nebulizer treatment, injecatble antibiotics and oral antifungal medication. He is now eating well and swimming normally.

However, we began to notice that the back end of his right lung looked more abnormal on x-rays. In this x-ray you can see that the back part of the right lung is whiter than it should be (I have have put an arrow there so you know where to look).
(Click image to enlarge.)It is conventional to view the x-ray as if you are looking at the turtle from the bottom. So his right side is on your left, and his left side is on your right.
The x-ray indicates that there is abnormal tissue in that part of the lung. We call this "consolidation," and it could mean that there is still an area of infection, or it could just be dense scar tissue that formed after the original infection had resolved. To further investigate this area, Orion had a computerized tomography (CT) scan at
Tufts Cummings School of Veterinary Medicine.
Orion gets a CT scan with the help of Tufts staff. (Click to enlarge.)The first CT image shows Orion as if you are looking at him from the front, and again his right is on your left, and his left is on your right. The first image shows the normal front part of the lungs, which are the two dark areas in the top of his body:
Normal lung (Click to Enlarge)The second image shows the consolidated (white) right lung, compared to the normal (black) air-filled left lung:
Abnormal lung (Click to enlarge.)Based on results of the CT scan, we decided to perform a lung biopsy on March 4 to determine whether there was still infection within the consolidated tissue. We used measurements from the CT scan to determine the precise location for the biopsy. Because the lung is just below the top shell or carapace, we can biopsy the lung by making a small hole through the shell, and inserting instruments into the lung.
Orion is anesthetized and a hole is made in the shell
(Click photos to enlarge)
Orion was put under anesthesia, then had a small hole (5 mm diameter) drilled through the shell. We then penetrated the lung to take a swab to test for bacteria and fungus.
Then, we inserted an endoscope into the lung.

A sample of Orion's lung is taken (left) and an endoscope takes images of the lung (right).
(Click photos to enlarge)The endoscope has a camera that allows us to view the inside of the lung on a video screen:

This photo shows a close up image inside the lung, as seen using the scope.

There are several small brown nodules in the lung (arrows are pointing to the nodules) which may be areas of current or past infection. The scope also has ports through which we can introduce instruments to take tissue samples or
biopsies.
Here you can see some of the tissue that we collected:

When the surgery was done, we sealed the hole with antibiotic paste, a small bandage, and waterproof epoxy:

We will remove the patch in a few weeks to see if it is healing OK. Orion recovered quickly from surgery and was back in his pool swimming happily by the end of the day.
The tissue biopsies have been sent to a lab for microscopic analysis (histopathology). This will allow us to see whether there is bacteria, fungus or other abnormal cellls. So far the culture tests have not found any evidence of infection. Orion can be seen on display in our Aquarium Medical Center. In fact, the entire surgery was perfromed on display in the Medical Center. So stop by to visit. You never know what cool stuff you might get to see!
Thanks to the staff at Tufts for assisting with Orion's CT scan.
- Dr. Innis
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Labels: CT scan, Dr. Innis, Kemp's ridley sea turtle, Orion, x-ray